scn2a (mouse, ib Search Results


scn2a  (Alomone Labs)
95
Alomone Labs scn2a
Immunoblots confirm the presence of several interactors in protein complexes that contain Kcc2. Kcc2 protein complexes were isolated from forebrain plasma membrane fractions of 8–12-week-old mice by immunoprecipitation, resolved by BN-PAGE and immunoblotted for components of the spectrin subnetwork. IPs were loaded adjacent to parallel IgG reactions to demonstrate specificity. Cntn1 and <t>Scn2a</t> were included as a further demonstration of band specificity, as proteomic analysis suggested specific enrichment in the 300 kDa and 800 kDa bands, respectively. Transparent overlays mark the location of each Kcc2 complex.
Scn2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Anti-SCN2A+(NaV1%2E2)+Antibody/pmc07643010-202-38-41
Average 95 stars, based on 1 article reviews
scn2a - by Bioz Stars, 2026-10
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anti-nav1.2 na+ channel antibody  (Antibodies Inc)
94
Antibodies Inc anti-nav1.2 na+ channel antibody
Immunoblots confirm the presence of several interactors in protein complexes that contain Kcc2. Kcc2 protein complexes were isolated from forebrain plasma membrane fractions of 8–12-week-old mice by immunoprecipitation, resolved by BN-PAGE and immunoblotted for components of the spectrin subnetwork. IPs were loaded adjacent to parallel IgG reactions to demonstrate specificity. Cntn1 and <t>Scn2a</t> were included as a further demonstration of band specificity, as proteomic analysis suggested specific enrichment in the 300 kDa and 800 kDa bands, respectively. Transparent overlays mark the location of each Kcc2 complex.
Anti Nav1.2 Na+ Channel Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Anti-Nav1%2E2+Na%2B+Channel+Antibody/custom%4075-024%4010%2E1101%2F2020%2E03%2E02%2E973859
Average 94 stars, based on 1 article reviews
anti-nav1.2 na+ channel antibody - by Bioz Stars, 2026-10
94/100 stars
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scn2a  (NeuroMab)
94
NeuroMab scn2a
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Scn2a, supplied by NeuroMab, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Anti-Nav1%2E2+Na%2B+Channel+Antibody/bio_rxiv__2020__03__02__973859-179-64-67
Average 94 stars, based on 1 article reviews
scn2a - by Bioz Stars, 2026-10
94/100 stars
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anti-kcc2 antibody  (Antibodies Inc)
94
Antibodies Inc anti-kcc2 antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Anti Kcc2 Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Anti-KCC2+Antibody/custom%4075-013%4033192291
Average 94 stars, based on 1 article reviews
anti-kcc2 antibody - by Bioz Stars, 2026-10
94/100 stars
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cdc2/cdk1 polyclonal antibody  (Bioss)
94
Bioss cdc2/cdk1 polyclonal antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Cdc2/Cdk1 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Cdc2%2FCDK1+Polyclonal+Antibody/custom%40bs-1341r%4010%2E1101%2F2020%2E04%2E30%2E071076
Average 94 stars, based on 1 article reviews
cdc2/cdk1 polyclonal antibody - by Bioz Stars, 2026-10
94/100 stars
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prrsv m protein polyclonal antibody  (Bioss)
92
Bioss prrsv m protein polyclonal antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Prrsv M Protein Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/PRRSV+M+protein+Polyclonal+Antibody/custom%40bs-4507r%4010%2E1101%2F2020%2E04%2E30%2E071076
Average 92 stars, based on 1 article reviews
prrsv m protein polyclonal antibody - by Bioz Stars, 2026-10
92/100 stars
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collagen 7 polyclonal antibody  (Bioss)
92
Bioss collagen 7 polyclonal antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Collagen 7 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Collagen+7+Polyclonal+Antibody/custom%40bs-1558r%4010%2E1101%2F2020%2E04%2E30%2E071076
Average 92 stars, based on 1 article reviews
collagen 7 polyclonal antibody - by Bioz Stars, 2026-10
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cd16 polyclonal antibody  (Bioss)
94
Bioss cd16 polyclonal antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Cd16 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/CD16+Polyclonal+Antibody/custom%40bs-6028r%4010%2E1101%2F2020%2E04%2E30%2E071076
Average 94 stars, based on 1 article reviews
cd16 polyclonal antibody - by Bioz Stars, 2026-10
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8-ohdg polyclonal antibody  (Bioss)
96
Bioss 8-ohdg polyclonal antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
8 Ohdg Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/8-OHdG+Polyclonal+Antibody/custom%40bs-1278r%4010%2E1101%2F2020%2E04%2E30%2E071076
Average 96 stars, based on 1 article reviews
8-ohdg polyclonal antibody - by Bioz Stars, 2026-10
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pan cytokeratin polyclonal antibody  (Bioss)
94
Bioss pan cytokeratin polyclonal antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Pan Cytokeratin Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/pan+Cytokeratin+Polyclonal+Antibody/custom%40bs-1712r%4010%2E1101%2F2020%2E04%2E30%2E071076
Average 94 stars, based on 1 article reviews
pan cytokeratin polyclonal antibody - by Bioz Stars, 2026-10
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acetyl coa carboxylase antibody  (Bioss)
94
Bioss acetyl coa carboxylase antibody
A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, <t>SCN2A,</t> SHANK3, SPTAN1 and SPTBN1.
Acetyl Coa Carboxylase Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Acetyl+CoA+Carboxylase+Antibody/custom%40bs-2745r%4010%2E1101%2F2020%2E04%2E30%2E071076
Average 94 stars, based on 1 article reviews
acetyl coa carboxylase antibody - by Bioz Stars, 2026-10
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ank2  (Bioss)
90
Bioss ank2
A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, <t>Ank2,</t> Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).
Ank2, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scn2a+(mouse%2C+ib/Ankyrin+B+Polyclonal+Antibody/bio_rxiv__2020__04__30__071076-188-18-21
Average 90 stars, based on 1 article reviews
ank2 - by Bioz Stars, 2026-10
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Image Search Results


Immunoblots confirm the presence of several interactors in protein complexes that contain Kcc2. Kcc2 protein complexes were isolated from forebrain plasma membrane fractions of 8–12-week-old mice by immunoprecipitation, resolved by BN-PAGE and immunoblotted for components of the spectrin subnetwork. IPs were loaded adjacent to parallel IgG reactions to demonstrate specificity. Cntn1 and Scn2a were included as a further demonstration of band specificity, as proteomic analysis suggested specific enrichment in the 300 kDa and 800 kDa bands, respectively. Transparent overlays mark the location of each Kcc2 complex.

Journal: Frontiers in Molecular Neuroscience

Article Title: Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes

doi: 10.3389/fnmol.2020.563091

Figure Lengend Snippet: Immunoblots confirm the presence of several interactors in protein complexes that contain Kcc2. Kcc2 protein complexes were isolated from forebrain plasma membrane fractions of 8–12-week-old mice by immunoprecipitation, resolved by BN-PAGE and immunoblotted for components of the spectrin subnetwork. IPs were loaded adjacent to parallel IgG reactions to demonstrate specificity. Cntn1 and Scn2a were included as a further demonstration of band specificity, as proteomic analysis suggested specific enrichment in the 300 kDa and 800 kDa bands, respectively. Transparent overlays mark the location of each Kcc2 complex.

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (Icc): Cntn1 (rabbit, IB, Abcam Ab66265), Itpr1 (rabbit, IB/Icc, Alomone Acc-019), Kcc2 (mouse, IP/Icc, NeuroMab 75-013), Kcc2 (rabbit, IB/Icc, Millipore 07-432), Scn2a (mouse, Icc, NeuroMab 75-024), Scn2a (rabbit, IB, Alomone ASC-002), Sptan1 (mouse, Icc, Abcam Ab11755), Sptan1 (rabbit, IB, Cell Signaling 21225), Sptbn1 (rabbit, IB/Icc, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026), vGAT (guinea pig, Icc, SYSY 131004), GABA γ2 (mouse, Icc, SYSY 224011), GFP (chicken, Icc, Abcam Ab13970).

Techniques: Western Blot, Isolation, Clinical Proteomics, Membrane, Immunoprecipitation

Components of the spectrin subnetwork co-localize with Kcc2 in several neuronal cellular compartments. Primary cultured neurons from P0 pups were infected with CamKII-AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin subnetwork; Sptan1, Sptbn1, Itpr1, and Scn2a. Co-localization was measured using line scans (white lines) and represent the normalized fluorescent intensity of single pixels (approximately 1 μm) on dendrites ( n = 3). Scale bars are 10 μm for large images and 5 μm for cropped images.

Journal: Frontiers in Molecular Neuroscience

Article Title: Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes

doi: 10.3389/fnmol.2020.563091

Figure Lengend Snippet: Components of the spectrin subnetwork co-localize with Kcc2 in several neuronal cellular compartments. Primary cultured neurons from P0 pups were infected with CamKII-AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin subnetwork; Sptan1, Sptbn1, Itpr1, and Scn2a. Co-localization was measured using line scans (white lines) and represent the normalized fluorescent intensity of single pixels (approximately 1 μm) on dendrites ( n = 3). Scale bars are 10 μm for large images and 5 μm for cropped images.

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (Icc): Cntn1 (rabbit, IB, Abcam Ab66265), Itpr1 (rabbit, IB/Icc, Alomone Acc-019), Kcc2 (mouse, IP/Icc, NeuroMab 75-013), Kcc2 (rabbit, IB/Icc, Millipore 07-432), Scn2a (mouse, Icc, NeuroMab 75-024), Scn2a (rabbit, IB, Alomone ASC-002), Sptan1 (mouse, Icc, Abcam Ab11755), Sptan1 (rabbit, IB, Cell Signaling 21225), Sptbn1 (rabbit, IB/Icc, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026), vGAT (guinea pig, Icc, SYSY 131004), GABA γ2 (mouse, Icc, SYSY 224011), GFP (chicken, Icc, Abcam Ab13970).

Techniques: Cell Culture, Infection

A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. KCC2 protein complexes were isolated from forebrain plasma membrane fractions of 8-12-week-old mice, resolved by BN-PAGE and immunoblotted for selected high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1.

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (ICC): ANK2 (mouse, ICC, Invitrogen 33-3700), ANK2 (rabbit, IB, Bioss BS-6967R-TR), ANK3 (mouse, ICC, Neuromab 75-146), ANK3 (rabbit, IB, Synaptic Systems 386003), CNTN1 (rabbit, IB/ICC, Abcam Ab66265), ITPR1 (rabbit, IB/ICC, Alomone ACC-019), KCC2 (mouse, IP/ICC, Neuromab 75-013), KCC2 (rabbit, IB/ICC, Millipore 07-432), NCKAP1 (rabbit, IB, Abcam 126061), NCKAP1 (rabbit, ICC, Sigma HPA020449), SCN2A (mouse, ICC, Neuromab 75-024), SCN2A (rabbit, IB, Alomone ASC-002), SHANK3 (rabbit, IB/ICC, Alomone APZ-013), SPTAN1 (mouse, ICC, Abcam Ab11755), SPTAN1 (rabbit, IB, Cell Signaling 21225), SPTBN1 (rabbit, IB/ICC, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Isolation, Clinical Proteomics, Membrane

A. Primary cultured neurons from P1 pups were infected with CAMKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for KCC2 and high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1 (n=3).

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Primary cultured neurons from P1 pups were infected with CAMKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for KCC2 and high risk ASD/Epi risk gene products; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1 (n=3).

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (ICC): ANK2 (mouse, ICC, Invitrogen 33-3700), ANK2 (rabbit, IB, Bioss BS-6967R-TR), ANK3 (mouse, ICC, Neuromab 75-146), ANK3 (rabbit, IB, Synaptic Systems 386003), CNTN1 (rabbit, IB/ICC, Abcam Ab66265), ITPR1 (rabbit, IB/ICC, Alomone ACC-019), KCC2 (mouse, IP/ICC, Neuromab 75-013), KCC2 (rabbit, IB/ICC, Millipore 07-432), NCKAP1 (rabbit, IB, Abcam 126061), NCKAP1 (rabbit, ICC, Sigma HPA020449), SCN2A (mouse, ICC, Neuromab 75-024), SCN2A (rabbit, IB, Alomone ASC-002), SHANK3 (rabbit, IB/ICC, Alomone APZ-013), SPTAN1 (mouse, ICC, Abcam Ab11755), SPTAN1 (rabbit, IB, Cell Signaling 21225), SPTBN1 (rabbit, IB/ICC, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Cell Culture, Infection

A. Total forebrain lysates and plasma membrane lysates were resolved by SDS-PAGE and immunoblotted for selected high risk ASD/Epi risk gene product; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1. B. The expression levels for each protein were quantified using densitometry and normalized to α-Tubulin loading controls (n=3). KCC2 (p=0.037), ANK3-190 (p=0.0014), CNTN1 (p=0.011), and ITPR1 (p=0.016) were significantly reduced in plasma membrane fractions.

Journal: bioRxiv

Article Title: The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products

doi: 10.1101/2020.03.02.973859

Figure Lengend Snippet: A. Total forebrain lysates and plasma membrane lysates were resolved by SDS-PAGE and immunoblotted for selected high risk ASD/Epi risk gene product; ANK2, ANK3, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1 and SPTBN1. B. The expression levels for each protein were quantified using densitometry and normalized to α-Tubulin loading controls (n=3). KCC2 (p=0.037), ANK3-190 (p=0.0014), CNTN1 (p=0.011), and ITPR1 (p=0.016) were significantly reduced in plasma membrane fractions.

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (ICC): ANK2 (mouse, ICC, Invitrogen 33-3700), ANK2 (rabbit, IB, Bioss BS-6967R-TR), ANK3 (mouse, ICC, Neuromab 75-146), ANK3 (rabbit, IB, Synaptic Systems 386003), CNTN1 (rabbit, IB/ICC, Abcam Ab66265), ITPR1 (rabbit, IB/ICC, Alomone ACC-019), KCC2 (mouse, IP/ICC, Neuromab 75-013), KCC2 (rabbit, IB/ICC, Millipore 07-432), NCKAP1 (rabbit, IB, Abcam 126061), NCKAP1 (rabbit, ICC, Sigma HPA020449), SCN2A (mouse, ICC, Neuromab 75-024), SCN2A (rabbit, IB, Alomone ASC-002), SHANK3 (rabbit, IB/ICC, Alomone APZ-013), SPTAN1 (mouse, ICC, Abcam Ab11755), SPTAN1 (rabbit, IB, Cell Signaling 21225), SPTBN1 (rabbit, IB/ICC, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Clinical Proteomics, Membrane, SDS Page, Expressing

A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, Ank2, Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).

Journal: bioRxiv

Article Title: Isolation and characterization of multi-protein complexes enriched in the K-Cl co-transporter 2 from brain plasma membranes

doi: 10.1101/2020.04.30.071076

Figure Lengend Snippet: A. Primary cultured neurons from P1 pups were infected with CamKII AAV-GFP at DIV 3 (to visualize cell morphology and to identify excitatory neurons) and fixed at DIV 21. The cells were immunostained for Kcc2 and components of the spectrin/ankyrin subnetwork; Sptan1, Sptbn1, Ank2, Itpr1. Colocalization was measured using line scans (white lines), and represent the normalised fluorescent intensity of single pixels (approximately 1 μm) on dendrites (n=3).

Article Snippet: The following antibodies were used for immunoprecipitation (IP), immunoblot (IB), or immunocytochemistry (Icc): Ank2 (mouse, Icc, Invitrogen 33-3700), Ank2 (rabbit, IB, Bioss BS-6967R-TR), Cntn1 (rabbit, IB), Abcam Ab66265), Itpr1 (rabbit, IB/Icc, Alomone Acc-019), Kcc2 (mouse, IP/Icc, Neuromab 75-013), Kcc2 (rabbit, IB/Icc, Millipore 07-432), Scn2a (mouse, Neuromab 75-024), Scn2a (rabbit, IB, Alomone ASC-002), Sptan1 (mouse, Icc, Abcam Ab11755), Sptan1 (rabbit, IB, Cell Signaling 21225), Sptbn1 (rabbit, IB/Icc, Abcam Ab72239), α-Tubulin (mouse, IB, Sigma T9026).

Techniques: Cell Culture, Infection